Composite

Part:BBa_K4273020:Design

Designed by: Su Junzhe   Group: iGEM22_LINKS_China   (2022-09-30)


pTDH3-Np5598-tTDH1-pPGK1-NlmysD-tPGK1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3423
    Illegal XhoI site found at 2075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2321
    Illegal BsaI.rc site found at 3433


Design Notes

To be functional in Saccharomyces cerevisiae


Source

cyanobacteria N. punctiforme and Nostoc linkia NIES-25

References